The 2021 Workshop directed by Daniel A. Peterson is offered on August 2-16, 2021. The course is reorganized to be held virtually. The purpose of this workshop is to provide a comprehensive background in the theory and practice of modern histological preparation and microscopic analysis for researchers in biomedical science. The workshop provides practical instruction in all aspects of microscopic analysis including experimental design, specimen preparation, use of fluorescence and confocal microscopes, quantitative histology using design-based stereology, and image preparation for scientific publication. Sessions on Rigor and Reproducibility have been added in response to the recent NIH criteria for grant funding. Other new sessions address tissue clearing, light sheet microscopy, handling of large image data sets, and use of artificial intelligence.

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